Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H

Two-photon fluorescence microscopy of corneal riboflavin absorption.

PURPOSE To correct for attenuation in two-photon fluorescence (TPF) measurements of riboflavin absorption in porcine corneas. METHODS Two-photon fluorescence imaging of riboflavin was performed using excitation at a wavelength of 890 nm, with fluorescence signal detected between 525 and 650 nm. TPF signal attenuation was demonstrated by imaging from either side of a uniformly soaked corneoscl...

Two Photon Absorption Laser Induced Fluorescence for Fusion Class Plasmas

Two Photon Absorption Laser Induced Fluorescence for Fusion Class Plasmas

Vibration and two-photon absorption

A treatment of nuclear vibrational motions in two-photon absorption is laid out in detail. Perturbation theory is used to develop working formulas for diatomic molecules and tested for the X S – A P transition of carbon monoxide. The results are compared with the exact numerical values. Certain vibrational contributions, not previously recognized, are found to make an important contribution to ...

Dynamics of two-photon-induced three-photon absorption in nanosecond, picosecond, and femtosecond regimes.

Based on five-level rate-equation theory, we develop the laser-pulse-duration dependence of two-photon-absorption-induced singlet and triplet excited-state absorptions (ESAs). We present analytical expressions for the effective three-photon absorption coefficients caused by both singlet and triplet ESAs under the pulsed excitation on time scales from femtoseconds to microseconds. We demonstrate...

Two-photon Fluorescence Endomicroscopy